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aflii restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs aflii restriction enzyme
    Aflii Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 556 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aflii+restriction+enzyme/AflII/pmc13127484-23-0-4
    Average 97 stars, based on 556 article reviews
    aflii restriction enzyme - by Bioz Stars, 2026-09
    97/100 stars

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    other:

    Article Title:
    Article Snippet: Homozygosity was confirmed using the following primers: 5'-CCACTGCAAACCACACATGT-3'; 5'- GGCCCTCTATAAGATCCCGG-3', and the size of the DNA fragments was quantified after digestion with the AflII restriction enzyme (#R0520, New England Biolab).

    Knock-Out:

    Article Title: Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation
    Article Snippet: .. To generate ATG13 knockout (KO) in HEK293 GFP-LC3 cells, CRISPR guide RNA (gRNA) forward and reverse sequences (Table S2) with overhangs for ligation into AflII restriction site were annealed and the resulting 100 base pair double-strand DNA fragment was cloned into the gRNA Cloning Vector (Addgene, 41824; deposited by Dr George Church) linearized with AflII restriction enzyme (NEB, R0520) using the Gibson Assembly® Master Mix (NEB, E2611). .. The recombinant ATG13 gRNA vector was co-transfected with a Cas9 expression vector (Addgene, 43861; deposited by Dr Keith Joung) in HEK293 GFP-LC3 cells.

    CRISPR:

    Article Title: Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation
    Article Snippet: .. To generate ATG13 knockout (KO) in HEK293 GFP-LC3 cells, CRISPR guide RNA (gRNA) forward and reverse sequences (Table S2) with overhangs for ligation into AflII restriction site were annealed and the resulting 100 base pair double-strand DNA fragment was cloned into the gRNA Cloning Vector (Addgene, 41824; deposited by Dr George Church) linearized with AflII restriction enzyme (NEB, R0520) using the Gibson Assembly® Master Mix (NEB, E2611). .. The recombinant ATG13 gRNA vector was co-transfected with a Cas9 expression vector (Addgene, 43861; deposited by Dr Keith Joung) in HEK293 GFP-LC3 cells.

    Ligation:

    Article Title: Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation
    Article Snippet: .. To generate ATG13 knockout (KO) in HEK293 GFP-LC3 cells, CRISPR guide RNA (gRNA) forward and reverse sequences (Table S2) with overhangs for ligation into AflII restriction site were annealed and the resulting 100 base pair double-strand DNA fragment was cloned into the gRNA Cloning Vector (Addgene, 41824; deposited by Dr George Church) linearized with AflII restriction enzyme (NEB, R0520) using the Gibson Assembly® Master Mix (NEB, E2611). .. The recombinant ATG13 gRNA vector was co-transfected with a Cas9 expression vector (Addgene, 43861; deposited by Dr Keith Joung) in HEK293 GFP-LC3 cells.

    Clone Assay:

    Article Title: Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation
    Article Snippet: .. To generate ATG13 knockout (KO) in HEK293 GFP-LC3 cells, CRISPR guide RNA (gRNA) forward and reverse sequences (Table S2) with overhangs for ligation into AflII restriction site were annealed and the resulting 100 base pair double-strand DNA fragment was cloned into the gRNA Cloning Vector (Addgene, 41824; deposited by Dr George Church) linearized with AflII restriction enzyme (NEB, R0520) using the Gibson Assembly® Master Mix (NEB, E2611). .. The recombinant ATG13 gRNA vector was co-transfected with a Cas9 expression vector (Addgene, 43861; deposited by Dr Keith Joung) in HEK293 GFP-LC3 cells.

    Cloning:

    Article Title: Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation
    Article Snippet: .. To generate ATG13 knockout (KO) in HEK293 GFP-LC3 cells, CRISPR guide RNA (gRNA) forward and reverse sequences (Table S2) with overhangs for ligation into AflII restriction site were annealed and the resulting 100 base pair double-strand DNA fragment was cloned into the gRNA Cloning Vector (Addgene, 41824; deposited by Dr George Church) linearized with AflII restriction enzyme (NEB, R0520) using the Gibson Assembly® Master Mix (NEB, E2611). .. The recombinant ATG13 gRNA vector was co-transfected with a Cas9 expression vector (Addgene, 43861; deposited by Dr Keith Joung) in HEK293 GFP-LC3 cells.

    Purification:

    Article Title: Dinoflagellate Phosphopantetheinyl Transferase (PPTase) and Thiolation Domain Interactions Characterized Using a Modified Indigoidine Synthesizing Reporter
    Article Snippet: .. This was purified, digested with AflII restriction enzyme from New England Biolabs, agarose gel purified, and combined and ligated in the same manner as the HindIII products resulting in “BpsA_insert1” ( ). ..

    Article Title: Dinoflagellate Phosphopantetheinyl Transferase (PPTase) and Thiolation Domain Interactions Characterized Using a Modified Indigoidine Synthesizing Reporter.
    Article Snippet: .. This was purified, digested with AflII restriction enzyme from New England Biolabs, agarose gel purified, and combined and ligated in the same manner as the HindIII products resulting in “BpsA_insert1” (Figure 1). ..

    Agarose Gel Electrophoresis:

    Article Title: Dinoflagellate Phosphopantetheinyl Transferase (PPTase) and Thiolation Domain Interactions Characterized Using a Modified Indigoidine Synthesizing Reporter
    Article Snippet: .. This was purified, digested with AflII restriction enzyme from New England Biolabs, agarose gel purified, and combined and ligated in the same manner as the HindIII products resulting in “BpsA_insert1” ( ). ..

    Article Title: Dinoflagellate Phosphopantetheinyl Transferase (PPTase) and Thiolation Domain Interactions Characterized Using a Modified Indigoidine Synthesizing Reporter.
    Article Snippet: .. This was purified, digested with AflII restriction enzyme from New England Biolabs, agarose gel purified, and combined and ligated in the same manner as the HindIII products resulting in “BpsA_insert1” (Figure 1). ..



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